Sentence examples for based on bisulfite conversion from inspiring English sources

To maximize the capacity for long-read sequencing, sample preparation was optimized based on bisulfite conversion and PCR amplification.

Since EDMA is not based on bisulfite conversion and sequencing, it does not provide a relative value of the extent of DNA methylation.

These pioneering studies were based on bisulfite conversion in human H1 embryonic stem cells, IMR90 fetal lung fibroblasts, and mature sperm cells [ 25].

In this study, we investigate the suitability of a range of methods based on bisulfite conversion and restriction enzyme digestion for accurate quantification of methylated DNA copies.

We note that the approach used for DNA methylation profiling is based on bisulfite conversion and therefore cannot distinguish between 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC).

Other methods based on bisulfite conversion measure both methylated and unmethylated DNA and lose sensitivity when the percentage of cells whose DNA is methylated in a certain region is lower than the noise-to-signal ratio of the method.

All these techniques are based on DNA bisulfite conversion.

MassARRAY® quantitative methylation analysis (Sequenom Inc). is based on the bisulfite conversion biochemistry followed by PCR and base-specific cleavage.

Like many other DNA methylation assays, this platform is based on sodium bisulfite conversion of DNA [ 11].

Quality control was also conducted at the sample level, based on the bisulfite conversion ratio and call rate rates (based on a detection p-value of 0.05).

To evaluate DNA methylation, the gold-standard method is based on sodium bisulfite conversion [ 14], in which unmethylated cytosines are converted into uracils.