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With the recent descriptions of the new species B. rhizoxinica and B. endofungorum, which are considered endosymbiotic bacteria in Rhizopus microsporus fungi, we now identify seven of these clinical isolates as B. rhizoxinica and one as B. endofungorum based on biochemical testing, 16s rRNA, and DNA-DNA hybridization results.
Underreporting of human cases is likely because identification of S. iniae is based on biochemical testing of isolates with commercial kits; the use of kits is associated with problems because S. iniae is not listed in commercial or clinical databases, and many atypical strains are assigned low matches (1, 4 ).
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Scleral exudates showed thin acid fast branching filaments under microscope and culture revealed Nocardia asteroides (identified based on biochemical tests).
Based on biochemical tests such as urease production, hydrolysis of casein, xanthine, tyrosin, and growth in gelatine, one of the isolates was identified as N. asteroids.
All B. pseudomallei isolates were previously characterized based on biochemical tests as well as by 16S rRNA sequencing [18].
Further characterization and identification has been traditionally based on biochemical tests and fatty acid methylester (FAME) analysis [106], [107].
This tool was designed for automated clustering and identification of Enterobacteriaceae based on biochemical test results.
All B. pseudomallei isolates were previously characterized based on biochemical tests as well as by 16 S rRNA sequencing [ 50].
The subtyping of enterococci on PhP-FS plates is based on biochemical tests with 24 different reagents, and was performed according to the manufacturer's instructions.
Using computer-generated synthetic data and some real data, we have demonstrated that BioCluster has high accuracy in clustering and identifying enterobacterial species based on biochemical test data.
Pure cultures of clinical isolates were identified based on biochemical test systems (API 20E, BioMerieux, France) following the instructions of the vendor.
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