Routine cultures initially revealed suspected Granulicatella/Abiotrophia species based on biochemical phenotype, good growth on chocolate agar, and colony satellitosis on sheep blood agar within beta-hemolysis zones of co-inoculated Staphylococcus aureus (Figure 1B,C,D).
Typing schemes based on biochemical and other phenotypic methods are dependent on many factors, including plastic bacterial phenotypes and the often low consistency of inter-laboratory testing [ 11].
If the observed in vitro stimulation were related to upstream TSS shifts at ADH1 observed in tfg alleles, then compromise of the TL might be expected to be epistatic to tfg phenotypes based on biochemical results.
KEGG pathways, which are built based on biochemical reactions and may provide biological explanations for phenotype variation, present the order of 100 genes, which corresponds to roughly d = 0.01 in Figure 1E (9684 genes times 0.01).
Nineteen Lactobacillus spp. were isolated and subsequently identified based on biochemical and molecular methods.
BNICE (Biochemical Network Integrated Computational Explorer), a computational approach for the discovery of novel biochemical pathways that is based on biochemical transformations, overcomes many of the current limitations.
Instead, based on biochemical data on the effect of mutations on NAT2 acetylator activity, it is plausible that in the ancestral genetic background, all slow-causing mutations had an equivalent effect on phenotype and fitness.
Based on biochemical and genetics studies, three alternative AsA biosynthetic pathways have been proposed (Figure 1A).
These genome-wide approaches based on biochemical purifications had to be confirmed by in situ analyses.
Species identification was based on biochemical profiling.
