Sentence examples for based on base call from inspiring English sources
A consensus sequence of the aligned contigs is produced, which can be masked based on base call proportion and coverage depth; single nucleotide polymorphisms (SNPs) can be masked similarly using separate user specifications.
Remaining reads were trimmed to remove low-quality bases; removing 5 bases from the 5′ end, 7 bases from the 3′ end, and again trimming the 3′ end based on base call quality scores.
This includes the deduplication of clonal reads, filtering based on base call quality, identification of potential bisulfite conversion errors and most notably the detection of single nucleotide variants (SNVs) that would affect methylation calls, the latter of which is until now only being performed by Bis-SNP.
Specifically, for each sample, we (i) trimmed reads to a length of 99 bases, (ii) excluded reads shorter than 99 bases, and (iii) excluded reads with expected error (a measure of read quality in USEARCH based on base call quality and read length) > 0.5.
Knowledge is based on forgetting.
Everyone on base calls him Sam.
We ignored the last 25 bp of each read from the VR1-5B strand and the two pools of selected segregants based on the base calling error rate estimated from unique alignments.
A custom script implementing strict quality filters based on the provided base call quality scores was used to trim sequences reads prior to assembly.
The extensive data base for this bat is based on search calls that are more evenly distributed over distances than the other bats in the guild.
Thus, using MAS, we first compared genotypes based on detection calls, and second based on the actual expression intensities of each probe set, filtered by detection call as suggested by previous studies [ 23, 24].
After alignment, sequence variants were called based on quantitative thresholds; bases called with a non wild-type frequenon wild-typere attributed to noise, 30% to 70% are called heterozygous changes, and 90% to 100% are called homozygous changes.
