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We thresholded images based on background fluorescence and measured total length of objects above background.
Gating exclusions were based on background fluorescence of the plasmid 99 PUR JM111 EGFP, an L1 construct containing two point mutations in ORF1 that completely abolish retrotransposition; 10,000 single-cell events per sample were gated and analyzed using CellQuest Pro (v.5.2).
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Cortical GFP− cells were isolated from Tie2GFP+ mice with gating based on background GFP fluorescence from wild type FvB mice.
The exposures estimated here were based on background PCB concentrations.
The edge of the cell could easily be identified based on either background fluorescence or that due to VSVG/KV4.2.
People form their opinions based on backgrounds and experiences.
The hybridization quality of each target area was based on fluorescence intensity background and signal to noise ratio, as described in detail at.
This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime.
Detection p values on the Goldengate Cancer Panel are calculated based on fluorescence signal at a probe compared with background fluorescence and represent one minus the probability) that a signal is stronger than background fluorescence.
9– 13 Detection modalities are based on fluorescence microscopic cytology, 8– 11 fluorescence spectrophotometry 12 and flow cytometry.
There were only two studies based on Asian background and one based on African background.
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