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As subphenotypes of AR based on asthma status are possible, we also performed secondary GWAS stratified by asthma status.
Importantly, we conducted environmental monitoring in the same manner for all study participants and did not differ it based on asthma status.
In the RCTs, asthma patients were divided evenly between the treatment and placebo or INCS alone arms, and no difference in outcomes based on asthma status was mentioned.
Trees were built to segregate subjects based on asthma status, and a minimum of 14 subjects per terminal leaf was specified (chosen to assure that leaves contained at least 10% of the total subject pool).
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The predetermined subgroups were based on asthma definition, the age of offspring, and study design.
Moreover, the guidelines provide criteria for determining asthma severity; and recommendations for pharmacological management of asthma are outlined according to a stepwise approach based on asthma severity.
The patients were divided into the following three groups based on asthma severity according to GINA [ 24]: (1) mild asthma (FEV1 ≥ 80%); (2) moderate asthma (FEV1 60-79%); and (3) severe asthma (FEV1 < 60%).
Covariates previously hypothesized to have an effect on asthma status (native status, breast-feeding, maternal smoking, income quintile, education quartile, maternal age, birth weight, and gestational length) were included in multivariate regressions based on statistical significance in bivariate regressions.
The physician examination was conducted once during study follow-up, and the children were classified as having current asthma or as not having current asthma based on their status at the time of the examination.
Stratified analyses were performed for subgroups based on gender, smoking status and respiratory health status (healthy vs self-reported current asthma or COPD assessed from spirometry).
The CASIS was highly successful in discriminating based on exacerbation status, patient-reported severity, patient-reported overall health, and clinician-reported severity (asthma group only).
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