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In principal, DNA methylation profiling could also be useful for discovery of biomarkers for detection of ovarian cancer, based on assays using sensitive PCR-based approaches for detection of methylated DNA [44] [51].
In these studies, the evaluation of cytoadhesion inhibition has been based on assays using purified non-placental sources of CSA bound to plastic dishes (static assays).
Based on assays using a 27-kDa antigen (CP23), the serum specimens from cases had a median response immunoglobulin (Ig) G level following clinical diagnosis (1,334) and a net response (433, change in IgG level from baseline) that were significantly higher than their respective control values (329 and -32, Wilcoxon p value = 0.01).
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Tolerance of adult plants to heat stress was based on assays used in Jenkins et al. (1997).
In our study we use a method of upscaling geological units, in this case based on assay data, using the continuous wavelet transform (CWT) and tessellation methods.
The experimental planning was based on eight assays using the 23 full factorial design combining three important variables for PDT: photosensitizer concentrations, incubation time of cells in HY solutions and employed light dose (λ = 590 ± 10 nm).
Based on activity assays using cell extracts, cyclic guanosine monophosphate adenosine monophosphate (cGAMP) was identified to be an activator of STING (Wu et al., 2013).
Quantification of the immunoprecipitated DNA was based on qPCR assays using primer pairs specific for either the upstream region of the β-globin gene, close to the promoter (region 1 in Figure 4B), or the 3' UTR (region 2 in Figure 4B).
During protein purification, the protein concentrations in the samples were determined based on Bradford assay using Quick Start™ Bradford Protein Assay Kit (Bio-Rad, USA) and bovine serum albumin (BSA) as standard.
However, principal component analysis of the matrices could be used to separate matrices based on the assay used (see Supplementary Figure S1B).
In vitro to in vivo correlations provides estimates of CLint based on in vitro assays using human liver microsomes, human hepatocytes, or recombinantly expressed enzymes.
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