Tables S1 and S2 summarize previously published viral load, immunological responses and experimental validation of escape mutations, and epitope sequence variation patterns determined through conventional sequencing, as well as some additional historical data that has not been previously published and some newly determined viral load data based on assays of stored samples.
Latent autoimmune diabetes in adults (LADA) subjects were excluded based on assays of GAD antibodies.
Relative fluorescent measurements were converted into yield of GUS as a percent of total soluble protein based on assays of GUS type VII-A from E. coli (Sigma-Aldrich) as a standard.
In diploid asexual populations, the relative fitness of mutators increased significantly (F1,24 = 192.3, p << 0.001) during the experimental propagation, rising to approximately 1.09 based on assays of three different mutator clones isolated at generation 180.
In any case, we did not find any evidence for synaptic innervation of immature adult-born granule cells at 3 days post-mitosis, based on assays of spontaneous events and the lack of response to high intensity 8 Hz stimulation synaptic responses.
Nevertheless, this parameter not only exhibited a close correlation with the glucose clamp-derived insulin sensitivity index [ 30], but it also was one of the best predictors of incident type 2 diabetes among surrogate estimates of IR based on assays of fasting insulin and glucose [ 8].
The assay is based on quantification of the degradation product of 2-deoxyribose by condensation with TBA.
The numbers of SNPs observed in CB4858 was based on the assay of 77% of the C. elegans reference genome (Hiller et al., 2008).
The total number of bases assayed in CB4856 was 4658922 (4.6465% of the C. elegans reference genome) while the number of SNPs observed in CB4858 was based on the assay of 77% of the C. elegans reference genome [ 11].
The diagnosis of GD1 was usually based on the assay of acid β-glucosidase activity and/or genotyping of GBA.
