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The study is based on appropriate staining methods and protocols developed in our laboratory.
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THE postmodern art movement of the 1980's was based on appropriating or borrowing paintings, unchanged.
The interpretation of cytoplasmic PAK1 expression was based on staining intensity and staining extent.
Antibody specificity was confirmed based on negative staining with an appropriate non-immune antibody, a concentration-dependent titration of staining intensity, a lack of extracellular staining, and specific competition of antibody binding by a pRKIP peptide (see Figure S1 in Additional file 1).
DIPEN staining was scored from 0 to 3 based on amount of staining, where 0 = no staining and 3 = maximal staining, as previously described [ 35].
EpCAM staining was evaluated using Allred scoring based on staining intensity and number of cells staining positively.
Nuclear immunoreactivity for PPAR γ was based on staining intensity and graded as 0, no staining; 1, mild-to-moderate staining; and 2, strong staining.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com