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The workshop participants – experts from academia, regulatory authorities, a scientific animal welfare organization, and the fish vaccine industry – agreed that efforts should be undertaken to replace the vaccination-challenge batch potency testing with tests based on antigen quantification or antibody response tests.
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DiscoTope [ 10] predicted antigenic determinants based on antigen 3D structures.
Yao et al. tested predictions based on antigen protein structures.
Similarly, estimates based on TMT quantification were poorly correlated with estimates based on 100% chemical acetylation (Appendix Fig S2B).
Traditional tests are based on cryoprecipitate quantification after prolonged (2 21 days) cold incubation of serum samples.
Copy number of DYZ1 was calculated based on absolute quantification method using qPCR.
The quantification of mRNA levels was based on the relative quantification method (2-ΔΔCt) [ 24].
While important to select a cancer cell-specific antigen, the prediction of total efficacy of ADCs based on the antigen expression level remains elusive (Damelin et. al., 2015).
The hepatitis B vaccine currently in use is based on an antigen produced in yeast, Professor Mason said.
As a result, a growing number of significant statements based on quantification can now be made.
Fig. 6 Divergent quantitative diagnosis based on method of quantification.
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