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Currently, there are several on-going projects to develop a VL test based on antigen detection, for example, the latex agglutination test detecting a urinary leishmanial antigen.
Conventional diagnosis of these causative agents is based on antigen detection and electron microscopy.
We discuss the limitations of IFA, debate the value of other methods based on antigen detection and nucleic acid amplification, and outline recommendations for future study.
Several ADAMTS13 assays are available today based on antigen detection and activity [ 15, 17, 25, 38], showing the presence of the protease (by enzyme-linked immunosorbent assay, ELISA) and its bioactivity in normal plasma and the lack of protease and activity in the plasma from patients with congenital TTP.
The work on antipeptide antibodies is relevant to the development of peptide-based immunogens both for active antibody-mediated immunization (e.g., as peptide-based vaccines) and to produce antibodies either for passive immunization (e.g., for antibody-mediated immunoprophylaxis or immunotherapy) or for immunodiagnostics based on antigen detection.
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Several biomarkers are currently tested with this strategy, either based on antigen-antibody detection or on fungal DNA detection in serum or blood.
Diagnostics based on serology, antigen detection or DNA are available but have limitations.
The prevalence of H. pylori based on stool antigen detection was only 6.3%.
Point-of-care (POC) tests for diagnosing schistosomiasis include tests based on circulating antigen detection and urine reagent strip tests.
Pre-emptive therapy based on pp65 antigen detection in PBL is associated with a reduction in the incidence of CMV disease in allogeneic SCT recipients [ 11].
The sensitivity and specificity of these assays was higher than individual assays based on direct antigen detection and standard PCR against a range of control templates and in several hundred clinical specimens.
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