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To further ensure that reads along exons did not originate from promoter regions or transcription end sites, we removed all entries in which the central exon was within 1 kb of any annotated transcription start or end site, based on annotations in the knownGenes table.
Significantly shared GO terms among the selected proteins from the Protoarray screen were identified with the Generic Gene Ontology (GO) Term Finder at the Lewis-Sigler Institute at Princeton University (release 27-Jan-2009; http://go.princeton.edu/cgi-bin/GOTermFinder, [50]) based on annotations in the Saccharomyces cerevisiae Genome Database (SGD).
The classifications were based on annotations in the Human Protein Reference Database (HPRD) [ 27].
The first two genes, Glyma06g08290 and Glyma04g08220 are oleosins (based on annotations in the Dana Farber tentative consensus sequences).
We also examined the distribution of DHSs relative to known non-coding RNAs (ncRNAs), based on annotations in WormBase (WS140) [ 8] and data from our lab [ 14].
were collected and curated based on annotations in PSI-MOD (Montecchi-Palazzi et al., 2008), RESID (Garavelli, 2004) and RCSB PDB (Berman et al., 2000).
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The region was also reported in [39], which is characterized by a high frequency of repetitive DNA (based on annotation in the Nipponbare genome (http://rice.plantbiology.msu.edu/) and it has low SNP polymorphism in all subpopulations except aus.
The target sequences included genomic regions containing the entire candidate genes, 5kb before transcription start site, and 2kb after the transcription end site, based on annotation in NCBI Build 35.
Note that these proteins do not act in isolation, but the GeneGo graphic shows the essential elements of the network based on annotation in GeneGo's MetaCore database.
Both sequences in the alignment were then trimmed to the extent of the human ORF based on annotation in the RefSeq or GenBank entry.
There are 80 drug/gene target combinations (Additional file 4) based on annotation in the GeneGo MetaDrug [ 25] database (14 genes crossed with 34 drugs).
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