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Putative metabolic pathways were assigned to genes based on annotations from the manually curated KEGG database.
Organelle targeting for transcript products was done based on annotations from the databases of JGI, NCBI, and ENSEMBL.
Self-prediction in Blast2GO (annotation based on annotations from the query sequence) was unavoidable, since dataset sequences cannot be removed from the database.
The first channel, called knowledge, is based on annotations from UniProtKB (1), MGI (2), SGD (3), FlyBase (4) and WormBase (5).
Based on annotations from both barley and rice we observed that this region contains a family GH9 enzyme and several transcription factors, which could plausibly regulate (1,3 1,4 -β-glucan 1,3 1,4 -β-glucan 1,3 1,4 -β-glucan
We first assembled two data sets: 1) the set of yeast open reading frames (ORFs) annotated as "integral to membrane" in the Saccharomyces Genome Database (Cherry et al. 1998) and 2) the set of membrane proteins of known 3D structure from the Protein Data Bank (Berman et al. 2000) that are divided into TM and EM regions based on annotations from the MPtopo database (Jayasinghe et al. 2001).
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Over-representation analysis of TF target sets was performed with Fisher's test based on annotation from TRANSFAC [ 42].
We report the design of an extended set of targets for capturing the complete human exome, based on annotation from the GENCODE consortium.
Based on annotations derived from Arabidopsis, 2128 hormones related genes were found on the cDNA microarray, about 3.5% of which (69) were differentially expressed in DGAT transgenic lines.
Variant consequences were obtained with SnpEff v3.1h (63) based on annotations extracted from the human and vertebrate analysis and annotation internal database taking the GRCm38 C57BL/6J mouse as the reference genome.
Based on annotations retrieved from mirBase (Griffiths-Jones et al., 2008), gtRNAdb (Chan and Lowe, 2009), Ensembl and Refseq, we identify intervals of known ncRNA classes in published deep sequencing data and benchmark our scoring system based on its ability to reassign an interval to its correct class, after its class label has been removed.
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