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The protocol is based on an affinity purification step followed by a strong cation-exchange chromatography and a refolding procedure adapted to epidermal growth factor domains and also to the high tendency of the protein to precipitate in concentrated solutions.
The Z-score of a ligand against a protein pocket is calculated based on an affinity profile of the binding pocket (Wang et al. 2012a).
The aforementioned observations and reservations were primarily based on analyses of three experimental studies of yeast proteins, two using Y2H screens [7], [9] and another using a HT mass spectrometric protein complex identification (HMS-PCI) technique [6], which is based on an affinity purification procedure.
The ProteoSeek™ Albumin/Ig Removal Kit (Thermo-Fisher, Bonn, Germany) was applied for albumin and Ig depletion, as based on an affinity resin column.
However, it should be noted that results based on an affinity chromatography approach indicate only a tendency of proteins to associate under physiological-like conditions and thus, they may lead to higher number of false positives.
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Hiring experts would quickly point out that most hiring mistakes are made when we select someone based on an emotional affinity rather than objective, rational appraisal of the candidate's skills.
The expression cassettes of the most successful vectors are based on a tandem affinity purification tag consisting of an octahistidine tag followed by the myosin motor domain tag.
In this paper, a patch-based super-resolution (SR) method is proposed to hallucinate facial images, where the image patches are selected and weighted based on a multilateral affinity function (MAF).
Here, we newly designed substrate channeling modules in Saccharomyces cerevisiae based on a high affinity interaction between dockerin and cohesin domains, which is a key process in the formation of cellulosome structure.
For example, WoW players are engaged not only within the game but they also converge in fan sites or forum sites to further interact, discuss, and analyze WoW based on a shared affinity for the game.
One such approach is based on a fluorescent affinity sensor comprised of concanavalin A (ConA).
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