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Molecular characterization of field strains is currently mostly based on amplification of the CYP51 gene followed by classic Sanger sequencing.
A conventional PCR assay based on amplification of the ORFV B2L gene was developed to detect many recognized Parapoxvirus species (Gallina et al. 2006; Sullivan et al. 1994).
In this paper, a method for visual attentional selection in mobile robots is proposed, based on amplification of the selected stimulus.
Therefore, a molecular method for identification of S. Weltevreden in clinical isolates based on amplification of a specific DNA sequence present in its genome is proposed.
In addition, RT-PCR based on amplification of gag sequences could be used to quantify RNA genomic sequences in supernatants from infected cells.
This study describes a duplex PCR assay developed for the detection of CcBV, based on amplification of the lef-8 gene (211 bp) of CcBV and the E75 gene (105 bp) of C. crangon as an internal amplification control.
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These reports are all based on amplification methods of two successive rounds of amplification using 3' priming, and in some cases three successive rounds [6].
A published PCR assay based on amplification and sequencing of a region of the 16S rRNA gene (rrs) 3 was used to test all water samples.
Traditional surveys of environmental prokaryotic communities are based on amplification and cloning of 16S rRNA genes prior to sequence analysis.
Several recent studies based on amplification and sequencing of the small subunit 18S ribosomal RNA gene fragment have revealed a great diversity of microeukaryotes [5], [21] [24].
This test is based on the amplification of a part of the L1 region of the HPV genome using the broad-spectrum SPF10-biotinylated primers.
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