Sentence examples for based on amplicon sequencing from inspiring English sources

To overcome this, we aimed to develop methodologies based on amplicon sequencing that involve the incorporation of barcoded amplification tags (BATs) into PCR products.

Recent studies have also provided nucleotide polymorphism information for P. mugo based on amplicon sequencing and candidate gene studies [ 6, 19].

Therefore, based on amplicon sequencing, the microsatellite markers showing cross-transferability across millet and non-millet species were valid and, thus, have utility in comparative genome mapping studies in both millets and non-millets.

The analysis of the metagenomic database to assign ancient DNA sequences to bacterial classes and species was still a significant challenge, and it has been noted that the analysis of human gut bacteria using shotgun metagenomic approaches yielded lower diversity estimates than those based on amplicon sequencing of the 16S rRNA gene (Qin et al. 2010).

However, given the biased procedure used to select SNPs in silico, the biased representation of polymorphic sites within these contigs and the skewed distribution of distances between sites (half the pairs being at a distance < 250 bp), the observed pattern of short-distance LD (not shown) was not consistent with trends typically observed in conifers [ 39] based on amplicon sequencing.

A recent study based on amplicon deep sequencing demonstrated that among the EBV alleles, the 1.2 allele is dominant in MS patients compared to healthy control.

So far, there has been no proven method to extract the information on gene copy number aberrations based solely on amplicon sequencing.

The AmpliSeq-RNA technology adapted to high-throughput semiconductor sequencing allows robust transcript quantification based on amplicon frequency.

Standard assembly based on amplicon, with primer removal was performed.

Analysis based on direct amplicon sequencing showed the expected conserved 5´ end followed by ambiguous sequences that corresponded to the hypervariable central region of msp2, as anticipated based on the presence of >52 msp2 copies in the A. phagocytophilum HZ strain genome (34 ).

This is higher than the efficiency range reported (46 to 55%) earlier based on direct amplicon sequencing [ 10, 12].