Sentence examples for based on amplicon frequency from inspiring English sources

The AmpliSeq-RNA technology adapted to high-throughput semiconductor sequencing allows robust transcript quantification based on amplicon frequency.

Based on amplicon sequencing of 16S rRNA genes, we determined the relative abundances of bacterial strains retained on the plant after washing compared to the initial suspension (Supplementary Material and Methods, Supplementary Table 1; Fig. 3).

In another study based on amplicon sequencing approach, it was suggested that deletions from less than 200 kbps to larger than 2 Mbps had occurred in wheat irradiated at 50 Gy with the same LET33.

Standard assembly based on amplicon, with primer removal was performed.

Alignments for single amplicons ordered based on variant frequency can be saved in single files.

We have adapted a commercially available mRNA quantification assay (AmpliSeq-RNA) that measures mRNA abundance based on the frequency of PCR amplicons determined by high-throughput semiconductor sequencing.

Based on expected amplicon sizes and frequency distributions of sequence lengths in v108 of the Silva reference database, sequences were further limited to a range of 325 425 bp.

Second, our workflow does not rely on pre-defined thresholds based on intra-amplicon variant frequencies for identification of true alleles (such as T2), which could create a bias in allele calling by not detecting alleles with low or inconsistent intra-amplicon frequencies.

We reported allele frequencies based on the frequency of the majority allele.

Descattering based on high frequency illumination serves the same purpose.

We estimated the frequency of null alleles that would result in the restoration of Hardy Weinberg equilibrium and, based on this frequency, we calculated the number of samples that would be homozygous for the null allele at each locus (Table 1) and so not yield an amplicon.