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Results were detected based on amplicon binding of Sybr Green using quantitative RT-PCR and are representative of at least three independent experiments.
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Based on amplicon sequencing of 16S rRNA genes, we determined the relative abundances of bacterial strains retained on the plant after washing compared to the initial suspension (Supplementary Material and Methods, Supplementary Table 1; Fig. 3).
In another study based on amplicon sequencing approach, it was suggested that deletions from less than 200 kbps to larger than 2 Mbps had occurred in wheat irradiated at 50 Gy with the same LET33.
Standard assembly based on amplicon, with primer removal was performed.
The AmpliSeq-RNA technology adapted to high-throughput semiconductor sequencing allows robust transcript quantification based on amplicon frequency.
Campbell, N. R., Harmon, S. A. & Narum, S. R. Genotyping‐in‐Thousands by sequencing (GT‐seq): A cost effective SNP genotyping method based on custom amplicon sequencing.
The gels were documented using VersaDoc Imaging System Model3000 (Bio-Rad Laboratories, Inc. USA) and allelic pattern was scored based on the amplicon size.
Calculations were based on known amplicon size and concentration as determined with an Eppendorf BioPhotometer.
SESAME is a web application package that assists genotyping of multiplexed individuals for several markers based on NGS amplicon sequencing.
This is higher than the efficiency range reported (46 to 55%) earlier based on direct amplicon sequencing [ 10, 12].
Product detection, allele calling, and hg inference were based on the amplicon-characteristic melting-point temperatures obtained with on-line post-PCR fluorescent dissociation curve analysis (DCA).
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