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Gavin Naylor's 1992 study, based on allozyme sequences, upheld and further resolved this "ridge-backed" group.
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Both morphotypes found in European rivers were genetically characterized as two distinct genetic lineages based on allozymes and mitochondrial COI sequences.
Earlier attempts to address phylogenetic relationships among Cheirolophus species or between the genus and its closest relatives have been based on allozymes [ 20] or DNA sequences from the nuclear ribosomal ITS and ETS regions [ 21, 22].
The diploid parents of both polyploids are in well separated clades [14] and are not closely related based on ITS/ETS sequences, allozymes and other genetic markers [15], [16].
Previous molecular analyses of the Amphiumidae based on allozymes [23] and mitochondrial DNA sequences [24] found conflicting relationships, although both studies were based on limiting sampling.
Based on sequence homology, 13,317 sequences were categorized into 44 functional groups.
Based on sequence homology, 56,098 sequences were categorised into 45 functional groups.
Based on sequence homology, 15,633 sequences can be categorized into 48 functional groups.
Primers for PAQR genes were based on sequences from published goldfish sequences (indicated above).
A secondary structure based on sequence alignment and sequence covariations was predicted for each element.
Based on sequence homology, the 18,736 sequences were categorized into 53 functional groups.
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