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Here, we present a deterministic approach for clustering huge compound libraries based on all pairwise compound similarities.
Statistical analysis of results comparing different strains with their isogenic sarA and agr mutants and their sarA-complemented derivatives was done by ANOVA based on all pairwise comparisons.
2. Construction of a phylogenetic tree based on all pairwise distances, to show the relatedness between the involved individuals.
The co-expression network was constructed based on all pairwise biweight midcorrelation values raised to a power β equal to 18. Biweight midcorrelation is less susceptible to outliers than Pearson correlation [ 23].
We then constructed for each ion transporter family a position-dependent scoring profile as outlined in figure 1. Reference protein sequences had been obtained from the manually curated section of the UniProt database (Magrane 2011) and had been redundancy-reduced based on all pairwise alignments (Smith and Waterman 1981).
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A similarity matrix based on all-against all pairwise PSSM alignments were constructed and hierarchical 'bottom up'-clustering was performed using the R package hclust.
Hamming distances among the six final alleles (i.e., the number of amino acid mutations in which they differ) within versus between treatments are tested with one-tailed two-sample t-tests based on all possible pairwise distances.
Thus, the sorting of ORs by neighbor joining analysis into distinct families and subfamilies is supported by an analysis based on all possible pairwise identities.
For each reference set, we estimated the average SN, PPV, and CPU time (for estimating the alignment scores/probabilities) of different alignment schemes based on all possible pairwise sequence alignments: 943 alignments for the reference set RV11, 2,335 alignments for RV12, 50,062 alignments for RV20, 76,370 alignments for RV30, 23,445 alignments for RV40, and 7,538 alignments for RV50.
Figure 1: Principal coordinates analysis of 32 goat populations based on a pairwise FST matrix.
Given the PFGE types of all S. aureus isolates within each flock, expected flock-specific distributions of PFGE type pairs based on all possible random pairwise combinations of isolates were arranged.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com