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The region was selected based on alignments and propensity scales.

To validate these variations, the following approaches were performed based on alignments of all available Illumina reads for TM-1 and Hai7124 to the TM-1 (v2.1) and Hai7124 v1.11) genomes by BWA (version 0.7.15-r1140 62.

Consensus primers, degenerate primers and virus-specific primers, whose designs were based on alignments of available cherry flexivirus sequences, were tested to amplify viral genomic fragments of six CGRMV isolates and one CNRMV isolate.

We extracted the SNPs based on alignments between ESTs and the A. gambiae reference genome.

The evolutionary conservation of amino acid sites with mutations was analyzed using ConSurf[25], [51], [52], based on alignments shown in Fig. S2 and S3.

All 5' targeting sites were chosen based on alignments of partially sequenced field samples (Accession numbers EU787517.1 – EU787984.1, AY462581.1 – and62851.1) and the published 3d7 var gene sequences.

A number of models (100 each) were generated using Modeller 9v7 with classical approach [60] based on alignments from bSVRs and aSVRs of the target and template sequences.

Our initial inspection of these loci was based on alignments and analyses of Drosophila and vertebrate conservation available through the UCSC genome browser.

For instance, only networks based on alignments that cover at least the length of the domain in common and have greater than 30% sequence identity may be of interest.

General comparison was conducted based on alignments with known motifs in the reference database.

The SILVA seed alignments are based on alignments published by the ARB project in 2004.

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