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Detroit is still catering to traditional car enthusiasts, but future customers will be more purposeful car lovers who prioritize brands based on alignment with their own personal values.
Exons were predicted with BlastX searches and manual editing based on alignment with cDNA sequences and on the canonical splicing rules (see Figure S2B).
To date no known genes have been annotated in the vicinity of this chromosomal region based on alignment with Ensembl-Database.
In the case of the third fragment (281048), the missing region was identified by the use of translated alignments against the Ciona genomic regions where the missing region was expected to be found based on alignment with the human orthologous protein.
Proposed 5' start of RNAs was based on alignment with known 5' start sites of homologous RNAs.
A region of GBE-unique sequences (aa 405 443), rich in Gly/Ala residues, has been identified based on alignment with GH13 sequences (25) (Supplementary Material, Fig. S4B).
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General comparison was conducted based on alignments with known motifs in the reference database.
SNP calling was performed based on alignments with the horse reference sequence.
For all isolates, the tuf gene was sequenced with species determination based on alignments with reference isolates.
Start and stop codons were determined based on alignments with the corresponding genes and proteins of other mite species.
The similarity of the amino acid sequences was determined based on alignments with the most likely human or mouse orthologs.
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