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The ancestral allele was based on alignment to the chimpanzee sequence.
Scaffolds were also reordered based on alignment to consensus genome maps.
The methods described above are all based on alignment to a database of genes with known (pheno-) types.
Five scaffolds were reoriented in ChLGX based on alignment to the consensus genome maps (Fig. 8 a– b).
Cultivar-restricted SNPs are SNPs found exclusively in the two cultivars based on alignment to the reference genome.
A typical SNP and INDEL calling pipeline based on alignment to a reference is restricted by the reference.
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Based on alignments to a composite reference of both mouse and C. albicans transcriptomes (Methods), the fraction of mapped reads for host and pathogen was highly correlated with the size of the transcriptomes and percent of sorted cells for each subpopulation (e.g., 87% host and 13% fungus for macrophages infected with live C. albicans; Fig. 1c; Supplementary Figure 1B; Supplementary Data 1).
The complete gene structures were determined based on alignments to mouse RefSeq mRNAs or species-specific mRNA, when available.
Alignments were performed iteratively and each sequence was refined based on alignments to make complete Or, Gr, and Ir sequences for A. cerana.
Comparative models were built using RosettaCM (Song et al., 2013) based on alignments to homologous structures generated using HHsearch (Remmert et al., 2011).
The latter case would require scaffolding to resolve based on alignments to reference transcripts, but was not conducted in these assemblies.
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