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We performed two separate analyses of our conserved clusters, one based on alignment of nucleotide sequences and another based on alignment of predicted peptide sequences.
Phylogenetic analysis from MEP/carotenoid enzymes pathways was based on alignment of concatenated sequences from 29 plant species and one moss species.
Phylogenetic reconstruction from proteins codified by a set of 13 single copy genes identified by Duarte and co-workers [ 21] was based on alignment of concatenated protein sequences from 28 plant species and one moss species.
Based on alignment of available and newly generated partial 5′-UTR nucleotide sequences, one forward and two reverse primers were designed, which amplify a 104 bp PCR product.
In this study, we selected 10 divergent epitope candidates based on alignment of the EV71 and CVA16 P1 amino acid sequences using the Multalin interface page, and these epitopes are conserved among all subgenotypes of EV71.
In this study, based on alignment of 70 full-length HEV sequences of genotypes 1 4, a part of the ORF2/ORF3 overlapping region was found to be the best target region for PCR amplification of various HEV strains.
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To validate these variations, the following approaches were performed based on alignments of all available Illumina reads for TM-1 and Hai7124 to the TM-1 (v2.1) and Hai7124 v1.11) genomes by BWA (version 0.7.15-r1140 62.
All 5' targeting sites were chosen based on alignments of partially sequenced field samples (Accession numbers EU787517.1 – EU787984.1, AY462581.1 – and62851.1) and the published 3d7 var gene sequences.
This benchmark tree was constructed based on alignments of 70 universal single-copy genes.
Initially, we attempted an SNP discovery approach based on alignments of ESTs.
Described alternatively spliced isoforms are based on alignments of ESTs and cDNAs onto these gene models.
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