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Our results showed that genetic lineage of the 2006b was derived from the 1996 variant, whereas the 2006a variant was closer to the 2004 variant based on alignment in the E region of the capsid gene.
(A ) The CENP-M alignment identifies highly conserved residues (based on alignment in Figure 3 figure supplement 1, panel D ), a subset of which (asterisks) is exposed at the surface of CENP-M.
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Edition of sequences prior to IM analysis using the IMGC program and based on alignment presented in additional files 1, 2, 3, 4, 5 and 6.
Specifically, AssembleIrysCluster calculated three "-T" parameters (5e-10, 5e-09 and 5e-08) based on the estimated genome size, and adjusted molecule map stretch and estimated molecule map noise parameters based on alignment to in silico maps.
Generation of pairwise comparisons of complete genome sequences was based on alignment of basepairs in MAUVE (24 ), which enabled alignment of whole genome sequences despite rearrangements.
Second, the quantification of orthologous genes across species can only be an approximation based on alignment scores, and in distantly related species, this may introduce confounding of the data.
Distant families were split based on alignment quality as measured in the percentage of gaps and where the oldest node in the tree was identified as a duplication event rather than a speciation event.
Therefore, even for the pseudogenes, we have still calculated the synonymous and nonsynonymous rates separately, assuming a reading frame based on alignment to the other genes in the data set, and have indicated the pseudogenes in Fig. 2C.
Phylograms calculated based on alignments trimmed in Gblocks are given in Additional file 4. The phylogenetic tree inferred based on the complete corrected alignment was used for the data interpretation.
Five scaffolds were reoriented in ChLGX based on alignment to the consensus genome maps (Fig. 8 a– b).
The human exon coordinates were used to identify coordinates for orthologous exon or intron boundaries based on alignment with human exons as indicated in the UCSC multiple sequence alignment of 44 vertebrate genomes (hgdownload.cse.ucsc.edu/goldenPath/hg18/multiz44way/).ucsc.edu/goldenPath/hg18/multiz44way/
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