Sentence examples for based on affinity column from inspiring English sources
Total RNA was extracted from 200 µl of plasma using a commercially available kit based on affinity column purification (High-Pure Viral RNA Kit, Roche Diagnostics, Mannheim, Germany).
"The Facebook multicultural targeting solution is based on affinity, not ethnicity.
The software identifies distinctly bound sites based on affinity data.
The ProteoSeek™ Albumin/Ig Removal Kit (Thermo-Fisher, Bonn, Germany) was applied for albumin and Ig depletion, as based on an affinity resin column.
The soluble fusion protein dissolved in buffer 1 (10 mM Tris-HCl pH 7.5 containing 150 mM NaCl and 5 mM β-mercaptoethanol) was firstly purified by Amylose Resin column based on MBP affinity.
Percentages are based on column totals.
*Percentage calculation is based on column total [ 19] denominator.
Thirdly, an additional purification step, possibly based on immuno-affinity chromatography of the FLAG-tag (Einhauer and Jungbauer, 2001), might be required to remove endogenous C. albicans proteins that co-purify with GR reporters on the nickel columns.
The RNA molecules are then selcted based on their binding affinity to the target molecule, for example, by passing them through a target-immobilized affinity column.
Hybrid sTRAIL proteins (P-dTags) mainly as heterologous trimers were obtained by elution with a low concentration of imidazole based on different binding affinities of His with a nickel column.
P values were based on z-test of column proportions.
