Sentence examples for based on affinity chromatography from inspiring English sources
Finally, a one-step purification procedure based on affinity chromatography was implemented to isolate the rhBMP-2 dimer.
We compared several purification methods based on affinity chromatography for purification of proteins from cell culture supernatants: metal chelate affinity, strep-tag and immunopurification with a monoclonal antibody.
Therefore, we designed a system based on affinity chromatography and could separate two types of complexes by placing an affinity tag on mutated EF-Tu species.
The strategy described in our report provides a simple and effective method for the production of [S]-biotin in E. coli based on affinity chromatography.
This is especially true when used in combination with a technology to enrich low-abundant proteins, i.e. the Proteominer™ technology, which is based on affinity chromatography using a solid phase combinatorial peptide ligand library.
Based on one affinity chromatography, an optimized enrichment of high-affinity aptamers is obtained by physical segmentation of the affinity resin.
Now, a new technology based on zonal affinity chromatography, weak affinity chromatography (WAC), has been introduced which has remedied many of the problems with other technologies.
TRAIL fusion proteins were produced in HEK293 cells as published, and purified from supernatants first by immobilized metal affinity chromatography (IMAC) based on affinity of TRAIL to Ni-NTA-Agarose (Qiagen, Hilden, Germany).
The purification scheme for RM.Eco29kI was based on affinity (Ni-CAM, nickel chelate affinity matrix, Sigma) chromatography.
ZrO2 is another useful phosphopeptide enrichment prior to MS analysis and its principle is based on metal affinity chromatography such as IMAC and TiO2.
To identify platelet proteins that interact with the C terminus of SERT, we used a proteomic approach based on peptide affinity chromatography by using a synthetic peptide corresponding to the last 26 amino acids from the C-terminus (586 630) of SERT.
