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The emerging view from this study of gene organization and global mRNA expression demonstrates that mRNA classes based on abundance, function or co-regulation are dispersed throughout the chromosomes, which is consistent with a model where gene-specific mechanisms involving trans-acting factors are responsible for regulating mRNA levels in this protozoon.
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This data is particularly powerful when aligned with EST data from related tissues, as it allows sorting based on abundance and predicted gene function.
Expression level was based on abundance in an EST survey of ∼3400 sequences [10].
Models based on abundance operate very differently than models based on scarcity.
In that regard, they introduce a novel (and possibly quite sound) 'complexity hypothesis' based on abundance.
Distribution of assembled transcripts based on abundance (FPKM) and corresponding read density.
Significantly regulated proteins were grouped into 11 clusters based on abundance pattern across all replicates.
In addition, all raw sequence reads recovered from the fosmid library were also exported to MG-RAST (Project No. mgp7584, access via http://tare.medisin.ntnu.no/data/mg-oil-compare/) to profile microbial diversity and abundance based on phylogeny and function (Meyer et al. 2008).
In summary, the high spectral content of DOSI measurements can be used to create complementary contrast functions based on the abundance and disposition of intrinsic tissue chromophores.
Their low abundance was expected based on the proposed function of CsoS4A pentamers at the carboxysome vertices and on their low steady state transcript levels [10], [17].
Carboplatin dose was based on renal function.
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