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The inset of Fig. 2c shows PL spectra normalized based on absorption at 633 nm for samples "f" and "g" of the series (these samples have equal MB concentrations).
RT concentrations were determined spectrophotometrically based on absorption at 260 nm using a calculated extinction coefficient (261,610 M−1 cm−1).
RNA was quantified based on absorption at 260 nm.
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The RNA yield was measured on the Nanodrop (Thermo Scientific, Wilmington, Delaware, US) and purity was based on absorption values at 280 nm (protein) and 230 nm (detection of contaminating organics/proteins) using the following criteria: 260 nm/280 nm >1.8 and 260 nm/230 nm >1.8.
This is particularly important because, based on absorption coefficient values42, carrier generation from interband transitions in Cu2O is being achieved at absorption depths much higher than its carrier diffusion length.
Holotomographic reconstructions were created based on absorption and phase contrast.
Furthermore, gains in economic efficiency and technological progress based on absorption of existing, imported technologies have now to be increasingly replaced by local innovative efforts.
However, the resulting image is different as conventional imaging and histology are based on absorption (light shade - little absorption, dark shade - strong absorption), whereas OCT is based on reflectivity (light shade - weak reflectivity, dark shade - strong reflectivity).
PPG is based on light absorption at a specific length wave; the light absorption is proportional to hemoglobin tissue content [ 31].
Purity of the samples was determined to be > 95% based on the absorption at 220 nm in analytical liquid chromatography-mass spectrometry (LC-MS) using a Agilent 1200 system equipped with a Phenomenex Gemini C18, 1 × 100 mm column with the same Agilent 6520 Q-TOF detector (Supplementary Note 2, Supplementary Figure 1, Supplementary Tables 1-3).
After 5 min incubation, 50 µL of 1 M NaOH was added and the total flavonoids were calculated from a standard curve based on the absorption at 510 nm.
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