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The central element of our recent studies was an in vivo-visualization system based on a stably transformed tobacco BY-2 (TBY-2) cell line for monitoring the prenylation status of a GFP fusion protein.
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The assay is based on a human epithelial cell line stably expressing a p65-GFP fusion protein whose nuclear translocation can be detected by automated microscopy.
We attempted to improve the transient transfection conditions with a stably NICD-expressing cell line based on a retroviral expression system.
This assay, termed Locus Derepression (LDR), is based on a GFP reporter gene under the control of the cytomegalovirus (CMV) promoter stably integrated in the genome of C127 mouse mammary adenocarcinoma cells.
Based on a site-specific recombination strategy, a modified Flp-In system was successfully developed to generate stably expressing cell lines.
"Based on a T.R.U.
Based on a pinned tweet.
"Venus" is based on a Bonnard painting.
Based on a novel by Mikhail Bulgakov.
The argument is based on a dilemma.
"Based on a True Story" — Blake Shelton.
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