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To verify the occurrence of disulfide bridge breaking we have carried out the Ellman assay based on a reagent [5,5′-dithiobis- 2-nitrobenzoic acid) or DTNB] able to quantify the concentration of thiol groups in a sample [5,5′-dithiobis- 2-nitrobenzoic
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This works by comparing the proteins in diseased and healthy tissues, and in diseased tissues before and after treatment, to try to work out how diseases operate at a molecular level.ICAT is based on a molecular reagent that has three components.
Here we describe an affinity biotinylation technique based on a photoreactive biotin reagent.
For screening antioxidants, the methods based on a single relatively stable reagent such as DPPH· and ABTS·+ have most popular, because of their simple set-up and ease of control [ 4].
Genomic DNA was extracted from whole blood using a method based on an organic deproteinization reagent.
Recently, we introduced polymer-based metal ion affinity capture (PolyMAC), a soluble reagent based on a titanium(IV -functionalized PAMAM dendrIV -functionalizedtrated enhanced rePAMAMcibility andendrimervity.
The control of the process presented in the paper has been based on a fuzzy algorithm of the reagent dosage control as a function of the solids concentration in the feed and its flow rate.
Sizes of rHA multimers were estimated based on a standard curve generated using reagents from a gel filtration HMW calibration kit (Cat# 28-4038-42, GE Healthcare Piscataway, NJ).
Nitrite accumulation, an indicator of NO synthesis, was measured in culture media based on a diazotization reaction using the Griess reagent [ 28].
Many researchers have focused on metabolic substrates such as [F]fluoro-2-deoxy-2-glucose (FDG), choline, and amino acids, which have been reviewed by Bénard et al. These reagents were based on an altered tumor metabolism such as increased energy requirements and elevated protein synthesis.
Here, we described a modified nucleofection technology based on alternative reagents which owns a high transfection efficiency (>80%) and also a relatively easy operation procedures.
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