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More recently, Ponomarenko et al 16 proposed a structure-based method called ElliPro that approximates protein surface patches as ellipsoids and then scores the residues belonging to the surface patches based on a protrusion index, which is a sophisticated way of selecting the most exposed residues in the structure.
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The protrusion volume was calculated based on a grid with 0.2-Å spacing.
Membrane protrusion analysis was done using Image J software and based on a program described elsewhere [ 29] with slight modifications.
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Germination rates were scored based on radicle protrusion.
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