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Detection of DENV RNA was measured using RT-qPCR based on an assay protocol modified from Sadon and others.
HSVECs (human saphenous vein endothelial cells) were isolated on the day of surgery by standard collagenase digestion based on a modified version of the protocol described by Jaffe et al. [ 25].
This communication is based on a protocol.
New probes and primers were designed for the qPCR assays, and IMNV detection was based on a modified protocol.
For insolubility assessment, four brain samples for each age and genotype underwent sequential extraction in buffers of increasing stringency, based on a modified protocol previously described [ 43].
These definitions are based on a modified version from a publication by Ljungman et al [ 14].
In this paper, a hybrid ad hoc routing protocol, i.e., an advanced OLSR (AOLSR) protocol, is proposed based on a modified version of Dijkstra's algorithm.
Symptom-limited maximal treadmill exercise testing was performed based on a modified Balke protocol (15) as previously described (7, 9, 14).
Total DNA was extracted from frozen leaf tissue based on a slightly modified version of the Dellaporta protocol (our unpublished data).
At the Government Virus Unit in Hong Kong, microneutralization-positive sera were confirmed by a single radial hemolysis assay for H9N2 antibodies, based on a modified, previously described protocol (21 ).
The presented methodology is based on a consensus protocol on the events observed by robots.
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