Sentence examples for based on a mutant from inspiring English sources

For S. longifissa, the complete TR region was sequenced using specific primers that was designed based on a mutant poly C (8 continuous C) (Additional file 4) in one of the TR units.

In order to draw metabolic flux to the biosynthesis of ansamitocins more efficiently, heterologous phosphoglucomutase was subsequently overexpressed based on a mutant strain with combinational regulation of PP pathway and ED pathway.

Gareia-Pedrajas and Roncero (1996) reported a highly efficient transformation system in F. oxysporum including DNA transformation of protoplasts mediated by PEG and protoplasting based on a mutant of NR which was complementary to homologous nitl gene and on the autonomously replicating sequence (ARS) and telomeric sequences presence in the vector.

An effective complementation assay is based on a mutant with a measurable phenotype that is easy to score.

The molecular mechanism of the HaloTag system is based on a mutant bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous, in which Phe272 is substituted by His272.

Internal SNPs localized the mutation between 66F and 69C, based on a mutant recombinant that excludes the 69C−75F interval and a wild-type recombinant that excludes the region distal to 66F.

Here, we describe the design, synthesis and thermodynamic characterization of a protein catenane based on a dimeric mutant of the p53tet domain (M340E/L344K).

The design of the PIR reporter protein is based on a p53 R175H mutant which, in contrast to the short-lived, WT p53, has a significantly longer half-life (several hours), presumably due to its reduced susceptibility to proteasomal degradation [37].

We used gene essentiality data based on a transposon mutant library of S. pneumoniae [ 14] to test PhenoLink.

The general role of PDR transporters in the resistance to antifungal drugs was first characterized in yeast (S. cerevisiae) and a particular function in DON resistance was confirmed based on a yeast mutant carrying a knockout variant of the PDR5 transporter gene resulting in a non-natural hypersensitivity to DON [ 109].

Relative to the parental strain, all Ins strains under-accumulated 18S rRNA, while the levels of 25S rRNA did not seem to be affected, proving our screen based on an rps mutant to be small subunit specific.