Duan and Li proposed a spectrum handoff strategy in which the optimal spectrum band was chosen based on a multiplex criterion considering the estimated transmission time, the PU presence probability and the spectrum availability time [17].
DPO-PCR is based on a multiplex PCR using a DPO patented technology [ 8].
Briefly, this assay is based on a multiplex PCR using ten primer pairs targeting genes showing variable number of tandem repeats.
From November 2003, patients admitted for longer than 24 hours were screened systematically on ICU admission using the qMRSA test, which is based on a multiplex immunocapture-coupled PCR, to identify patients colonized with MRSA.
Detection of blaOXA was based on a multiplex PCR assay developed by Woodford et al. [ 12] with addition of blaOXA-48-like primers (OXA-48L 5′- GTGGGATGGACAGACGCG-3′ and OXA-48 LL 5′-CCACACATTATCATCAAGTTC-3′) using National Collection of Type Cultures (NTCT) 13442 K. pneumoniae as positive control for blaOXA-48.
To overcome these drawbacks, we developed a prnp typing method that is based on a 'multiplex amplification refractory mutation system' (ARMS) reaction.
Based on this, a multiplex PCR was designed that revealed a high prevalence of prophage and/or defective prophages within S. epidermidis strains.
The aim of this study was to develop a method for detecting antibiotic resistance (AR) genes in cheese based on a combination of multiplex PCR and a DNA microarray hybridisation system.
The aim of this study was to develop and validate an analytical method based on a mitochondrial DNA (mtDNA) multiplex PCR technique which would allow the early and specific identification, in one step, of C. daubneyi and F. hepatica infection in G. truncatula.
Serum samples were tested for 14 analytes (granulocyte macrophage colony-stimulating factor (GM-CSF), IL-1β, IL-4, IL-6, IL-10, IL-12p70, IL-13, IL-17, IL-23, IFN -γeron (IFN -γ, CCL2, CCL3, CCL4, and tumor necrosis factor (TNF -α using cusTNF -αsting services based on a flow cytometry bead-based musinglex immunoassay panel (provided by BioLegend, Incustomn Diego, CA).
A small number of newer emerging CE-marked PCR technologies are also currently available that can detect pathogen DNA rapidly in blood samples without the need for pre-culture, either using an all-pathogen approach without simultaneous speciation (eg, SepsiTest; Molzym GmbH, Bremen, Germany) or by using a multiplex approach based on a wide pathogen panel (eg, VYOO; SIRS-LAB GmbH, Jena, Germany).
