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The construction of two chimeric classical swine fever virus (CSFV) recombinants based on a marker virus constructed previously [J. Virol. 72 (1998) 5318 5322] is described.
This composition was evaluated from counts of white and red colonies based on a marker system that exploits the suppression of the ade1-14 allele that was present in all yeast strains used in this study.
To be an exhaustive search for QTLs, a genome scan should be based on a marker set that covers the entire genome.
However, marker order in bighorn sheep for that region appeared to be the same as in the IMF map based on a marker located only 2 cM away from TGLA53 in domestic sheep (CSAP01E).
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Neutral marker evolution was based on a similar marker number (177), and the same map positions as in linkage group 11 in R. Burrishoole.
For qALT6, foreground selection was based on a STS marker for ZmMATE1 and the SSR marker, umc1018, whereas for qALT5 we used only the STS marker for ZmMATE2.
The method is similar to that used by Tian et al. (2011) on maize except that our association analysis, like that of Lafarge et al. (2017), was based on a single marker model rather than a multiple marker model.
We also confirmed that the sex locus is situated downstream of SSR marker VVIB23, while previous studies, based on a lower marker density, had placed it upstream [ 17, 20].
There is one problem with monitoring approaches based on a single marker, a problem which has plagued physicians in oncology and related fields for decades: the heterogeneity of tumours.
"Our industry is not interested in any way, shape or form in discriminating based on a genetic marker".
Here we present an extensively evaluated and – to our current knowledge – specific TaqMan® PCR assay for the detection of B. anthracis based on a chromosomal marker.
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Justyna Jupowicz-Kozak
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