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Functional enrichment of genes associated with CNEs was carried out using the GREAT software58 with each CNE assigned to the genes with the nearest transcription start site and within 1 Mb in the zebrafish genome, and significantly enriched functional categories identified based on a hypergeometric test of genomic regions (false discovery rate (FDR) q value < 0.05).
To facilitate GO and KO analyses of query genes, we developed online tools to identify the enriched or depleted GO/KO terms within a query gene list based on a hypergeometric distribution.
This is highly significant when compared to a random assignment based on a hypergeometric distribution, which would yield, on average, only 24 out of 48 mice correctly classified.
It is based on a hypergeometric distribution.
An enrichment p value based on a hypergeometric test is calculated together with a p value corrected for multiple testing.
In both cases, a P value for each gene was calculated based on a hypergeometric distribution test (5% FDR).
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This intersect between genes predicted by both data sets was highly statistically significant (P < 7.422e-66, calculated based on an hypergeometric distribution analysis [ 31]).
Based on a classical hypergeometric test for Gene Ontology term enrichment, the FATIGO tool provides a t-test for cross-comparison enrichment analyses from two gene lists.
Recently, in myasthenia gravis Cao et al. [ 26] calculated the cross-talk between pathways, identified by pathway-enriched analysis based on a cumulative hypergeometric distribution.
Based on a tailed hypergeometric distribution analysis only pathways over-represented in the group of co-expressed genes (p [hyper] < 0.005) were retained.
The crosstalk between pathways was calculated based on a cumulative hypergeometric distribution using the following formula: (1) P = 1 − ∑ k = 0 x m k N − m n − k N n, where N is the total number of genes in the human genome, n is the number of genes in one pathway, m is the number of genes in a different pathway, and x is the number of genes that are common to both pathways.
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