The timing for sample collection was based on a dose-response curve (supplemental material, Results S1, Figure S1) as well as previous studies [47].
These seeds were germinated with and without the addition of smoke water (standard Themeda solution; diluted 1 : 500 000 based on a dose-response screening experiment reported in the electronic supplementary material, table S2) [ 5].
Data are incidence rate ratio (95% confidence interval) derived from the multivariate adjusted models described in the text based on a dose-response analysis with predefined categories of circulating 25- OH D and tertiles of dietary calcium intake.
Data are incidence rate ratio (95% confidence interval) derived from the multivariate adjusted models described in the text based on a dose-response analysis with pre-defined categories of circulating 25- OH D and sex specific categories of alcohol, and tertiles of retinol intake.
This hypothesis is based on a dose-dependent response according to which influenza mortality increased when healthy susceptible persons were exposed to a high infectious dose of the 1918 influenza virus.
The 50 µg dose of the inhibitor was chosen based on a previous dose response curve performed in our lab which showed it to be the most optimal and effective dose in inhibiting Rac GTPase activation in the hippocampal CA1 region following intracerebroventricular (icv) injection, and which did not display any significant behavioral side effects [7].
Estimates of additional lifetime from 45 years of occupational exposure (lifetime exposure) to 1 ppm derived using he Paustenbach et al. exposure matrix and best-fitting (quadratic) models ranged from 0.020 to 0.036 per thousand, whereas corresponding estimates based on a linear dose response ranged from 1.6 to 3.1 per thousand.
The IC50 (the dose needed to inhibit growth by 50%), was calculated based on a sigmoidal dose-response curve using the nls package in R (www.r-project.org) [37].
However, brief exposure to increased doses of luciferin before blastocyst transfer had no marked effect on implantation, and 50 µg luciferin/ml of KSOM was used in this study, based on a luciferin dose-response curve (maximum photon flux/blastocyst).
Due to differing transduction efficiencies among cell lines, each cell type was infected at an optimized MOI, based on pilot dose response studies conducted with the Ad/ GFPTET vector.
