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However, these methods are based on a confocal approach with all its limitations.
Later, equipping the detector with a second polycapillary lens, a new concept based on a confocal configuration was proposed.
Fluorescence lifetime imaging microscopy was performed with the use of an in-house developed confocal microscope based on a confocal microscope Olympus FluoView 300 (Olympus UK Ltd ,Watford, UK) coupled with a supercontinuum laser source (SC450, Fianium, Southampton, UK) [35].
This instrument, called the confocal scanning laser ophthalmoscope (SLO), was based on a confocal optical imaging system and a scanning laser light beam illuminating the retina.
Fluorescence lifetime imaging microscopy (FLIM): All fluorescence lifetime images were recorded on a home-built confocal microscopy platform based on a confocal microscope scanning unit (Olympus FluoView 300) coupled with an Olympus IX70 inverted microscope frame.
Dirckx et al. devised a method based on a confocal microscope by measuring the optical thickness which was related to the RI and the physical thickness of a sample [ 14].
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Based on a preliminary confocal analysis of five bright and five dim worms, we found that these activity loci were caused by extremely bright intestinal cells.
Higher resolution images were acquired with a custom-built video-rate two-photon microscope based on a Olympus BX50 confocal laser-scanning system, equipped with a titanium-sapphire femtosecond laser (Tsunami, Spectra-Physics) tuned to 780 nm, photomultiplier tubes for detection, and a 60× 1.10 NA water-immersion objective as previously described [40].
Our FMM system is based on an Olympus FV300 confocal microscope.
Here, we present the design and construction of a confocal endoscope based on a fiber bundle with 1.4-μm lateral resolution and 8-frames per second (fps) imaging speed.
Here we report about such development of an FMS system based on a white light spinning disk confocal microscope.
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