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Based on a bioinformatics approach, three human CD19-derived sequences were designed and inserted into a self-inactivated lentiviral vector backbone upstream of the GFP gene: S.CD19 (230 bp), M.CD19 (464 bp), and L.CD19 (1274 bp).
Based on a bioinformatics pipeline, we identified 176 new S. japonicum miRNAs, of which some exhibited a differential pattern of expression between the two stages.
Lamberti et al. confirmed serine and threonine phosphorylation of eEF1A, and suggested other likely Ser and Thr phosphorylation sites based on a bioinformatics study of eEF1A1 [32].
We have proposed the name PNG2 based on a bioinformatics analysis suggesting that it encodes a non-cytoplasmic (i.e. secreted or cell surface-located) peptide N-glycanase (peptide N-glycanasel-b-D-glucosaminyl)aspeptide-N4- N-acetyl-b-D-glucosaminyl
To investigate the extent that ER-based proteins affect the organisation of Drosophila tER-Golgi units, we designed a double screening strategy based on a bioinformatics pre-selection combined to a morphological RNAi screen.
The first RNA recombination of the JEV was proposed based on a bioinformatics analysis [ 17].
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Our approach, based on a bioinformatic analysis of CCG promoters, does not allow to distinguish core regulatory elements (E-boxes, ROR elements), input pathways (CREB elements), direct output (D-boxes) and secondary regulation (via hormones, nervous system, feeding, sleep-wake cycle or body temperature).
Based on a bioinformatic and mutational approach in yeast Liu and co-workers [ 30] suggested the co-evolution of Mbf1 with Tbp in Eukaryotes and Archaea.
The technique is based on a bioinformatic separation of the mouse and human transcriptomes from the initial mixed-species transcriptome resulting from sequencing an excised tumor/stroma specimen without prior cell sorting.
Based on a bioinformatic analysis of the cloned Hydra homologue PSR, Cikala and colleagues were the first to suggest that the protein might function as a 2OG-dependent dioxygenase that is capable of modifying unknown nuclear targets [ 57].
The restriction enzyme selection was based on a bioinformatic analysis of the DNA sequence of the target gene and chosen to be around 500 1000 bp away from the gene-specific primer-binding site.
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