The experimental approach in the present study was based on a bioassay-guided fractionation.
A topical, 5-dose bioassay with adult Melanoplus sanguinipes was conducted with doses bracketing the estimated LD50 for each strain, based on a preliminary bioassay.
There are two datasets shown in the Figure 1: 1> statistics for active compounds based on all bioassays (including primary, confirmatory); 2> statistics for active compounds based on confirmatory bioassays.
The variability of a bioassay based on a luminescent bacterium was examined as an example.
In an initial screening experiment, BNI activity in the exudates of 36 different rice genotypes was evaluated using a bioassay based on a recombinant Nitrosomonas strain.
Regarding the best EC conditions, toxicity, based on an Artemia salina bioassay, and biodegradability, based on the Zahn-Wellens test, were assessed in raw SLL effluents and after their treatment with EC and biological processes.
Results from the bioassay based on a double fluorochrome staining were worthy to be described because they provide a clue to the mode of action of one (18) of the most promising anticancer lead structures of the series.
The humanized h357 IgG1 was then stably expressed in a mammalian cell line and the purified antibody maintained the high antigen binding affinity as compared with the parental m357 based on a soluble TNF-α neutralization bioassay.
However, we used RPF values that are the most recently accepted estimates based on a comprehensive analysis of tumor bioassay data (U.S. EPA 2010).
The correct and specific functioning of this yeast glucocorticoid bioassay was studied by exposures to cortisol and other related compounds and critically compared to a GR-CALUX bioassay based on a human bone cell.
Using a recently developed GC bioassay based on a GC-responsive reporter construct in human Jurkat T-ALL cells, we found ~7-fold higher biological activity with dexamethasone than prednisolone.
