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Here, light sheet based microscopy would be an ideal solution for single molecule experiments in thick specimen [19], [20].
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Replacement of light microscopy with fluorescence microscopy would be one of the immediate options for improving TB case detection in high-burden settings.
Thus a negative malaria result diagnosed by microscopy would be more reliable than a positive result.
Cloneable labels for electron microscopy, equivalent to green fluorescent protein in fluorescence microscopy, would be a great help; first steps in this direction look promising [ 26].
Therefore, additional methodological approaches such as fluorescence lifetime imaging microscopy (FLIM) would be required.
One way we could do this would be by microscopy.
This approach would be a nonlinear variant of localization microscopy and localization microscopy could be given the optical sectioning capability of nonlinear microscopes.
Thus, cytosolic epitopes would be accessible for immunofluorescence microscopy in PFA/PBS fixed cells.
As 1.71 FTHOE would be required just for the microscopy, 3 4 staff would need to be employed for the ZN microscopy component of the work.
This would be readily evaluated by confocal microscopy in permeabilized cells.
Qdot photostability would be an asset in Fluorescence Speckle Microscopy [45], [46] applications and could permit tracking of actin dynamics in processes that depend on actomyosin based contraction.
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