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Amplification with T7 transcription better reflects the variation of the unamplified transcriptome than PCR based methods owing to the better representation of long transcripts.
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CMV QNAT, based most commonly on polymerase chain reaction (PCR), has largely replaced conventional methods owing to better overall performance, and clinical guidelines now recommend the use of these assays for CMV load monitoring in SOT and HSCT recipients to prevent or to manage CMV replication and disease [ 4, 5, 7, 8].
Biotechnological processes are notoriously difficult to control using conventional methods owing to their time-variant and non-linear characteristics.
On the other hand, the promising numerical results of filter methods owe to their non-monotonicity in a certain degree.
FIA based on electrochemical methods can be called most environmentally friendly analyzing method owing to less analyte consumption.
Gas chromatography is an important method owing to its speed, resolving power, and detector sensitivity.
Nevertheless, data transformation is needed in this method owing to the limited models that can be presented in linear forms.
Unfortunately most fracture fluids currently deployed are aqueous based owing to their ease of preparation and low cost.
In this context, source tracking of environmental pollutants and process modeling using biological based methods are becoming increasingly important, mainly owing to the accuracy and robustness of such techniques.
We did not apply the more sensitive counting ASE method C-BASE owing to time constraints and the laborious nature of this assay.
This method cannot be applied to larger knowledge bases owing to its quadratic space requirement in terms of RAM [6].
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