Mass spectrometry (MS -based MS -basedave becomethodsprimary techaveogy used to identify proteins.
Mass spectrometry-based methods have become increasingly important in structural biology — in particular for large and dynamic, even heterogeneous assemblies of biomolecules.
Compared with other fully automatic methods [ 14, 20– 22], SPM-based methods have become increasingly popular because they are easy to implement and exhibit good discriminative performance.
Furthermore, PCR-based methods have become increasingly reliable with the introduction of high-fidelity DNA polymerases, thus limiting the number of unwanted mutations in the final construct [ 19- 21].
Over the last decade bone-Pb measurements based on noninvasive in vivo X-ray fluorescence (XRF) methods have become increasingly accepted.
His methods have become famous.
Over the decades, her methods have become more cunning and more detailed.
Finally, sensitive microscopic methods have become more routine practice.
Radiological imaging methods have become digital over the last decade.
"The methods have become more subtle".
While many served their purpose, these methods have become passé.
