So far the antibody based methods for hepcidin quantification in human serum are either semi-quantitative [15], [16] or measure the prohormone whose correlation to the levels of hepcidin25 remains controversial [17], [18].
In this work, a harmonic analysis based method for linearity assessment and noise quantification in EIS measurements is presented, and validated both from an experimental point of view and from a theoretical point of view, for Tafelian systems.
To allow similar analysis of unopsonized phagocytosis of specific bacterial pathogens we have developed high-throughput fluorescent scanning cytometry-based methods for quantification of phagocytosis of S. aureus by adherent human alveolar-like macrophages in a 96-well microplate format.
In vitro test have shown potential for the use of RP-HPLC and NMR based methods, but only for the quantification of pure fractions of cyclotides, and showed limitations for spectrophotometric methods for these peptides [ 43].
The acylcarnitine platform is an UPLC-MS/MS based method that allows for the separation and quantification of several important isomers of acylcarnitine species as well as trimethylamine-N-oxide, choline, betaine, deoxycarnitine, and carnitine.
During the past years, novel mass spectrometry-based methods such as ICAT [2], SILAC [3] and iTRAQ [4] were developed for relative quantification.
Based on this, an electrochemical method for quantification of cypermethrin has been proposed that can detect cypermethrin down to 2 ppb.The work has opened up new possibilities for electrochemical study of detoxification processes carried out by GSH.
We describe a PCR method for quantification of microRNAs based on a single reverse transcription reaction for all microRNAs combined with real-time PCR with two, microRNA-specific DNA primers.
An external method for quantification of organochlorine compounds based on retention times was used.
Here, we report on an alternative method for quantification of secreted proteins based on the split GFP assay.
