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PEG was added to MS medium according to diffusion based method described by Girma and Krieg (1992).
These compounds showed satisfactory anti-HIV-1 IN activity by cell-free assay system [ 23] and the ELISA based method described by Tewtrakul et al. [ 27].
For GISH, total genomic DNA (gDNA) was extracted from young leaves of seedlings using the cetyltrimethylammonium bromide (CTAB) based method described by Murray and Thompson [ 42].
> Our evaluation of the success of the different barcode loci corresponds to the tree-based methods described by Ross et al. (2008).
Positive selection has been estimated among self-incompatibility alleles of several taxa using various methods [ 13, 15- 19], most commonly the maximum likelihood phylogenetic approaches first proposed by Nielsen and Yang [ 20] and more recently by coalescent-based methods described by Wilson and McVean [ 21].
The liquid culture conditions, sample collection and preparation, and analysis were based on methods described by [28].
From the DLP data, the ED in mSv was calculated based on methods described by Huda and colleagues (conversion factor = 0.0175) [13].
The calculation is based on methods described by Collett.
Exact 95% CIs were calculated based on methods described by Rothman and Boice (1979).
Target predictions were performed based on methods described by Schwab et al. (2005) [ 39].
Uptake assays in whole yeast cells were based on methods described by [ 37].
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