RMA-based methods (as used here) have superior low-level detection capability compared to GCOS-MAS5 [21]; however, to ensure that normalization method was not responsible for the observed correlations, we recomputed the cross-area correlations using MAS5-normalized data.
Therefore, fluorescence-based methods as used by Lin et al. may provide correct binding affinities.
As mentioned in the text, we also varied the threshold to the 90th or 99th percentile, and also tested the Poisson based methods as the one also used by Ernst et al. [ 19], with a significance threshold of P < 0.05 or P < 10-5.
The ply drop boundary optimisation is formulated using topology optimisation techniques and is thus solvable using gradient based methods as well.
In addition the use of intein based methods as well as the preparation of the solid support for Staudinger ligation often require reagents such as phosphines or thiophenols that are toxic and difficult to handle.
The phylogeny was constructed using a maximum likelihood based method as implemented in PhyML [ 72] using the JTT substitution model [ 73] with the default proportion of invariable sites (0.0) and rate heterogeneity between sites corrected by a gamma law (using the default gamma parameter of 1.0 and eight rate categories).
Furthermore, physiologically based demographic models (see "Methods") as used here could provide a rapid way of evaluating short-season cotton varieties and control strategies for cotton pests in the different regions of India and elsewhere (e.g., [89]).
Concentrations of PCB153 in pup livers on PND19 and abdominal fat in week 16 was analysed by Gas Chromatography/Mass Spectrometry (GC/MS) in SIM mode, based on the same methods as used to analyse the feeds [ 46, 47].
The MID of the LCQ and VAS were determined using anchor based methods using the GRCQ as described by Juniper [ 7].
The Multi-Level Wave Based Method is used as the prediction method.
Tree methods use sequence alignments and phylogenetic trees for predicting evolutionary relationships, while graph based methods use pairwise sequence similarity search methods (such as BLAST) to predict orthologs.
