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However, MI based methods as previously formulated, provide limited insight into the dynamic behavior of the system, and hence have limited use in predicting new observations a key property for estimating a model's relevance when the ground truth is unknown.
Gene prediction was done using a combination of ab initio and EST-alignment based methods as previously detailed [ 8, 25].
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The infection was quantified by a CFU (colony forming unit) based method as previously described [16], [23].
The levels of the NIS mRNA were assessed by a semi-quantitative RT-PCR based method, as previously described [ 12].
Genomic DNA was extracted from myoblasts and telomere length was measured by a qPCR based method as previously described by Mamchaoui et al. (Skeletal muscle 2011).
The high percentage reported by the latter group could be related to the study of a different population (lesbian women), to the use of culture methods better suited for L. crispatus and corresponds better with results obtained by non culture based methods, as reported previously [ 16, 22, 26, 27, 32, 36- 38].
C-terminal truncations were made by PCR-based methods as previously described [40].
MethylC-Seq libraries were generated by bisulfite-based methods as previously described.
However, these methods require images to be processed by dedicated software and are more complex to be implemented in clinical routine than RC-based methods, as previously discussed ((A3) Applicability) and also commented by Soret et al. [ 16].
Only patients with high resolution typing of HLA-A, B, C, DRB1, DQB1, DPA1 and DPB1, verified using appropriate DNA-based methods as previously described, were included in the study.
The endogenous genomic copies of DYN1, PAC1, NDL1, and NIP100 (encoding the dynein heavy chain, Lis1, Nudel, and dynactin subunit p150Glued orthologs, respectively) were deleted or modified using PCR-based methods as previously described (Longtine et al., 1998 ).
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