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Materials excluded from column porosity showed no fluorescence, indicating that UV-fluorescence based methods are unable to detect high mass materials.
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Similarity-based methods are unable to classify reads from unknown species without close references (which constitute the majority of reads).
Second, library-based methods are unable to identify new families that display no similarities with existing families.
Unfortunately, shrinkage-based methods are unable to provide unbiased effect estimates, and some of them (i.e., ridge regression) do not perform variable selection.
Because full-coalescent model-based methods are unable to computationally handle large amounts of data, these summary statistics provide essentially the only current option for generating species trees using hundreds of loci obtained from hundreds of taxa.
Further, a known limitation of bisulfite sequencing based methods is that the protocol is unable to distinguish difference between 5-methylcytosine and 5-hydroxymethylcytosine.
Traditional paper-based data collection methods are unable to capture clinical work complexity and communication patterns.
From previous work, we know that most state-of-the-art texture feature extraction methods are unable to cope even with minor blur degradations if the classifier's training stage is based on idealistic data.
Moreover, these methods are unable to elucidate the causes of poor PTA.
However, most of these methods are unable to handle problems with incomplete and uncertain data.
On the other hand, biological methods are unable to remove dyes from effluents effectively.
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