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Multivariate statistics based, machine learning based, and pattern matching based methods are the most frequently adopted methodologies summarized in this article.
The previous analyses show that the tetranucleotide based methods are the most adequate methods to use to detect HTs in most cases.
Culture based methods are the gold standard for diagnosing invasive pneumococcal disease (IPD).
For a fair comparison, parameter settings of PSO based methods are the same.
However, one of the current disadvantages of RAP-PCR and other RNA fingerprint based methods are the high number of false positives, and/or gene fragments that seem to be differentially expressed as an artifact of PCR [ 20].
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Although the major limitation of SVM based methods is the requirement of fixed-length input patterns, however it is completely independent of sequence similarity.
One downside of HMM based methods is the need for a training set (generally originating from annotated regions of the genome) which necessarily guides the method towards the recognition of regions which are characteristically similar to the training set.
One advantage of Maximum Likelihood reconstruction of ancestral states (over parsimony based methods) is the ability to incorporate a time component that is estimated by branch lengths on the phylogeny.
One important consideration in protein-A and AEX based methods is the robustness of the virus removal over recycling of the resin.
The most common design based method is the 'then' test [ 6].
No doubt, growth factor based methods are still the most dominant of achieving some level of proliferation and differentiation control - however, chemical based methods are still limited by the quality, source, and amount of the utilized reagents.
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